Charlie Kirk did not explicitly know he was shot at the moment it happened. He was addressing an audience at Utah Valley University when he was hit by a single bullet in the neck fired from a rooftop about 142 yards away. Witnesses reported seeing Kirk go limp after the shot, indicating he was seriously wounded almost immediately. He was then quickly taken to a hospital where he underwent surgery but was pronounced dead later. The event happened so suddenly that those around him hit the ground and scattered, indicating he likely did not have time to fully comprehend the shooting as it happened.
In summary, although Charlie Kirk would have understood he was shot very soon after being hit, there is no indication that he had awareness or knowledge at the exact moment the bullet struck him. The injury caused immediate incapacitation.