The distance between Jacksonville, Florida, and Orlando, Florida, is approximately 140 to 143 miles (about 225 to 227 kilometers) by road. The driving distance is around 141 miles (227 km), which typically takes about 2 to 2.5 hours depending on traffic and route taken

. The straight-line flight distance ("as the crow flies") between the two cities is about 125 miles (201 kilometers)

. Travel options include driving, bus, and train:

• Driving takes roughly 2 to 2.5 hours.
• Bus services (FlixBus, Greyhound) take around 2 hours 40 minutes to 3 hours 15 minutes.
• Amtrak train travel takes about 3 hours 15 minutes

In summary, the cities are about 140 miles apart by road and 125 miles by air, with driving time around 2 hours.