Skunks can spray multiple times, typically up to five or six times in a row before their glands are depleted. After using their spray, it usually takes about 7 to 10 days for the skunk to fully replenish its spray supply. Because they have a limited amount of spray, skunks tend to be reluctant to use it unless they feel truly threatened and will often give warning signs before spraying. In the wild, a skunk might spray only 1-2 times per year, but in higher-stress environments, such as near humans or predators, they might spray as often as once a month or a few times a week.

Summary:

• Maximum sprays per encounter: 5 to 6 times in quick succession.
• Replenishment time: About 7 to 10 days.
• Frequency in the wild: 1-2 times per year.
• Frequency in higher stress/human environments: Up to once a month or more.
• Skunks usually warn with behaviors before spraying.

This limited but effective defense mechanism is reserved for serious threats and helps conserve their precious spray resource.