To calculate the total magnification of a microscope, you use the following formula: Total Magnification = Magnification of Objective Lens × Magnification of Eyepiece Lens

• The objective lens is the lens closest to the specimen, and its magnification is usually marked on the lens (e.g., 4x, 10x, 40x, 100x).
• The eyepiece lens (ocular lens) is the lens closest to your eye, usually marked with its magnification (commonly 10x).

For example, if the objective lens is 40x and the eyepiece lens is 10x, the total magnification would be: 40 × 10 = 400x This means the image appears 400 times larger than the actual specimen

. Additionally, when measuring magnification from an image, the magnification can also be calculated by dividing the size of the image by the actual size of the specimen: Magnification = Image Size ÷ Actual Size This method is often used when you have a microscopic image and want to find the magnification or actual size of the specimen

. In summary:

• To find microscope magnification: multiply objective lens magnification by eyepiece magnification.
• To find magnification from an image: divide image size by actual specimen size.

Both methods are fundamental in microscopy calculations.