The gender of Mewtwo is a topic of debate among fans, and there are conflicting answers from different sources. Here are the main points from the search results:

• The original Mewtwo, created by Team Rocket in the first movie and Mewtwo Returns, was male.
• In the more recent movie, Mewtwo -- Prologue to Awakening, Mewtwo appears to be female, with a female voice actor.
• Mew, although genderless, is often thought of as being female.
• From an actual gameplay standpoint, Mewtwo is considered genderless.
• Some fans believe that there are two separate Mewtwos, one male and one female.

In conclusion, the gender of Mewtwo is not definitively established, and it may depend on the source or interpretation.