In microbiology, a colony is a group of bacteria, fungi, and other microorganisms grown on a solid agar medium. The cells plated on this medium grow to form a mass, which can then be duplicated for further use in the lab. Colony morphology is used to pick out a pure colony, which is a colony grown from a single parent cell. Morphology encompasses physical characteristics like form, margin shape, elevation, surface texture, opacity, and color. Observing the colony morphology can be done with the naked eye, or it can be imaged with a camera and image analysis software for automation. Microbial colonies are widely used in a wide range of research and to produce various proteins and enzymes. To obtain the total viable cell count, the number of colonies is multiplied by the sample’s dilution factor on the chosen agar medium. Colony morphology is an important skill used in the microbiology laboratory to identify microorganisms.