The purpose of using a water bath in isolating DNA is to increase the yield of DNA by slowing down the enzymes that could break the DNA strand. The warm water bath helps to denature the enzymes and increase the rate of reactions, which unzips the double helix and separates the DNA from the proteins. The warm water bath also helps to break down cell membranes and nuclear membranes, and to denature proteins that can interfere with DNA extraction. Water baths are commonly used in DNA extraction, protein purification, bacteria transformation, CRISPR, agar and agarose melting, and thawing embryos, sperm, and vaccines.