The ideal internal temperature for pork tenderloin when you take it out of the oven is 145°F. This is a safe internal temperature that will give you a much juicier, more tender piece of meat than the previously recommended temperature of 160°F. It is important to use a digital cooking thermometer to check the internal temperature of the pork tenderloin. After cooking, let the pork tenderloin rest for about 5 minutes before slicing. This will allow the juices to redistribute and make the meat more tender.