You should take a pregnancy test ideally from the first day of your missed period for the most reliable result. Some sensitive pregnancy tests can detect pregnancy hormone (hCG) a few days before your missed period, but testing too early may lead to a false negative because the hormone levels might not be high enough yet. For best accuracy, waiting a week after your missed period is recommended. If your menstrual cycle is irregular or you don't know when your period is due, it's best to take the test at least 21 days after unprotected sex. Also, the best time of day to take a urine pregnancy test is in the morning with your first urine because it contains the highest concentration of hCG, which improves the test’s accuracy. In summary:

• Take the test on the first day of a missed period or later.
• For more accurate results, wait a week after the missed period.
• If irregular cycles or unsure period date, test 21 days after unprotected sex.
• Morning urine is best for testing.

If early signs of pregnancy appear, you can test sooner but be aware of possible false negatives if done too early.